While "sterility" implies the destruction of free-living organisms which may grow within a sample, sterilization does not necessarily entail destruction of infectious matter.Prions are an example of an infectious agent that can survive sterilization by moist heat, depending on conditions.
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If used in the range 0 °C to 50 °C (32 °F to 122 °F), a check at the ice melting point or at ambient temperature will suffice for verification.
If used over a broader range, THG recommends combining inspection of historical calibration records with a check at 0 °C (32 °F) or at ambient temperature.
Four Methods for Thermometer Verification Comparison with another calibrated thermometer at ambient temperature For this measurement, you need to have another calibrated thermometer.
Heating an article is one of the earliest forms of sterilization practiced.
The various procedures used to perform moist heat sterilization process cause destruction of micro-organisms by denaturation of macromolecules.